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Description

Briefly, isolated cells (from culture plates or scrapings) were lysed in RIPA buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 0.1% SDS) or M-Per (Thermo Fisher Scientific, 78501) and supplemented with protease and phosphatase inhibitors (Thermo Fisher Scientific, 78443)

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9 During this period, Congress consistently encouraged the componentsand in particular the Services that accounted for the vast majority of SBIR awardsto promote commercialization

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"NMR has been shown in recent years to be a powerful tool for studying metabolites as they operate in living cells, so we felt it could work well as a noninvasive way to do the same for GSH and GSSG," says NIST research chemist Vytas Reipa

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10.3389/fnmol.2022.1079338 111 YuJ.ZhongB.ZhaoL.HouY.AiN.LuJ

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